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What if I used my old DS-160 for new interview appointment?

For each DS-160 submitted, it generates an unique barcode. if you have saved the earlier DS-160 data in .dat file format, you can use the same and will able to enter less information in your new DS-160, if that was the intention. Dont know how many days have passed, chances are some of the information might have changed, while you submit the new DS-160.